Year: 2018 | Month: April | Volume 8 | Issue 1
The Possible Role of Certain Glycosidase on Cow Reproduction
Abstract:
Glycosidase are associated with cumulus cells expansion, sperm capacitation, sperm oviductal epithelial cells interaction, sperm zona pellucida binding and polyspermy block. The aim of this review is to summarize the role of certain glycosidase (α-mannosidase - α-ΜΑΝ, β-N-acetyloglucosaminidase – β-NAGASE and β-galactosidase - β-GAL) on cow reproduction. The activity of certain glycosidase in: i) the cervical mucus after spontaneous or induced estrous and its relation to cow fertility, ii) the uterine luminal fluid after superovulation, iii) the follicular fluid and the maturation, fertilization or culture medium during IVM-IVF is presented in this review. Furthermore, it is mentioned if: a) the endometrium or oocytes or embryos release or use glycosidase during their development, b) the addition of certain glycosidase in culture medium affects embryo development, and c) glycosidase could be used as markers of embryo quality or superovulatory response (SR). Glycosidase activity was significantly lower in the cervical mucus of spontaneous estrous compared to induced estrous cows. A high superovulatory response is related to low β-NAGASE, probably because of the poor quality of embryos. The β-ΝAGASE affects negatively embryonic development when added to culture medium. COCs release β-NAGASE and use β-GAL during maturation. Embryos release β-NAGASE and α-MAN during their development, but they use only α-MAN. Degenerated embryos release less β-ΝAGASE and α-MAN compared to good embryos, whereas β-ΝAGASE seems to be related to retarded morulae. Glycosidase affects the developmental competence of oocytes collected from different sized follicles during IVF.
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